Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CDC73

Cell type

Cell type Class
Bone
Cell type
U2OS
Tissue
bone
Lineage
mesoderm
Description
osteosarcoma from the tibia of a 15 year old, J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma, viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses, mycoplasma contamination was detected and eliminated in 1972, (PMID: 6081590)

Attributes by original data submitter

Sample

source_name
osteosarcoma cell line
cell line
U2OS
tissue
osteosarcoma cell line
chip antibody
Parafibromin (rabbit, bethyl laboratories, A300-171A)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
crosslink (10 min), harvest, lysis of cells, fragment with sonifier libraries were prepared for sequencing using standard NEB protocols

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
22799678
Reads aligned (%)
98.2
Duplicates removed (%)
5.6
Number of peaks
983 (qval < 1E-05)

hg19

Number of total reads
22799678
Reads aligned (%)
97.8
Duplicates removed (%)
6.9
Number of peaks
971 (qval < 1E-05)

Base call quality data from DBCLS SRA