Antigen

Antigen Class

TFs and others

Antigen

CDC73

Cell type

Cell type Class

Bone

Cell type

U2OS

Tissue

bone

Lineage

mesoderm

Description

osteosarcoma from the tibia of a 15 year old, J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma, viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses, mycoplasma contamination was detected and eliminated in 1972, (PMID: 6081590)

Sample

source_name

osteosarcoma cell line

cell line

U2OS

tissue

osteosarcoma cell line

chip antibody

Parafibromin (rabbit, bethyl laboratories, A300-171A)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

crosslink (10 min), harvest, lysis of cells, fragment with sonifier libraries were prepared for sequencing using standard NEB protocols

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

22799678

Reads aligned (%)

98.2

Duplicates removed (%)

5.6

Number of peaks

983 (qval < 1E-05)

hg19

Number of total reads

22799678

Reads aligned (%)

97.8

Duplicates removed (%)

6.9

Number of peaks

971 (qval < 1E-05)